International Journal of Biotechnology and Microbiology

International Journal of Biotechnology and Microbiology


International Journal of Biotechnology and Microbiology
International Journal of Biotechnology and Microbiology
2022, Vol. 4, Issue 1
Serological and molecular comparison study for diagnosis of herpes simplex virus type 1 patients Infection in Mosul/Iraq

Ibtisam Raad Abdullah, Anmar Ahmed ALTaie

Herpes Simplex Virus-1 (HSV-1) have the quiescent nature (dormant and recurrent infections). Therefore it is common to give a false negative results in the diagnosis by using diagnosis methods such as serological methods such as Enzymed-Linked Immunosorbent Assay (ELISA) and molecular methods such as Polymerase Chain Reaction (PCR). The present study is conducted to investigate HSV-1 infections like cold sores, Herpetic Gingivostomatitis (HGS), Herpes Gladiatorum (HG), Eczema Herpeticum (EH), and HSV-Associated Erythema Multiforme (HAEM) from Al-Salam Teaching Hospital, Mosul during the period from 1/2/2021 to 1/10/2021. The tested patients fall within the age category of (1 to 60) years. One hundred swab samples were collected, only 22 and 7 samples (22%, 7%) were indicate positive for HSV-1 for Serological assay (Enzyme-Linked Immuno Sorbent Assay - ELISA) and molecular assay (Polymerase chain reaction - PCR) respectively. ELISA shows high percentage of HSV-1 (8%) in (36-45) year, while PCR shows percentage of HSV-1 (3%) in (11-25) year, (2%) in (26-35) year, and (2%) in (36-45) year. The sensitivity and specificity between ELISA and PCR were (0.93, 0.91) respectively.
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How to cite this article:
Ibtisam Raad Abdullah, Anmar Ahmed ALTaie. Serological and molecular comparison study for diagnosis of herpes simplex virus type 1 patients Infection in Mosul/Iraq. International Journal of Biotechnology and Microbiology, Volume 4, Issue 1, 2022, Pages 87-90
International Journal of Biotechnology and Microbiology