Screening Rose spp. from India for Apple mosaic virus and Prunus necrotic ringspot virus infection and designing a duplex RT- PCR assay for their simultaneous detection
Latief A Wani, Priyanka Jawa, Jawaid A Khan
During spring in the years 2017, 2018 and 2019, field surveys were conducted in the valley of Kashmir, New Delhi, Palampur (H.P), Khitoli (U.P) and Jhajra-Dehradun (U.K), to screen the rose species from India for Apple mosaic virus (ApMV) and Prunus necrotic ringspot virus (PNRSV) infection. A total of 236 symptomatic and non-symptomatic leaf samples were collected and subjected to RNA isolation followed by cDNA synthesis. The PCR amplification was carried by using CP gene specific primers for detection of ApMV and PNRSV. Two samples from Kashmir and three from Dehradun were found infected with ApMV, sharing > 96 % to 100 % sequence identity with CP of rose gene isolates from Poland and apple isolates from India. PNRSV was detected only in four samples from Kashmir sharing > 98 % to 99 % sequence identity with CP gene of apricot isolates from India and rose isolates from Poland. None of the samples from other sampling sites were found infected with neither of the virus, and no infected sample was found to have a mixed infection. The duplex PCR assay designed for ApMV and PNRSV detection was 100 % specific to their targets and could simultaneously detect them up to 1 ng / µl concentration. This assay is more reliable, specific, and sensitive for early detection of these viruses and is worth using in pant quarantine for disease management and control.
Latief A Wani, Priyanka Jawa, Jawaid A Khan. Screening Rose spp. from India for Apple mosaic virus and Prunus necrotic ringspot virus infection and designing a duplex RT- PCR assay for their simultaneous detection. International Journal of Biotechnology and Microbiology, Volume 4, Issue 1, 2022, Pages 77-86