The production of Indole acetic acid (IAA) is the main feature of rhizospheric bacteria that stimulates plant development. IAA is a frequent metabolic product of L-tryptophan metabolism. The present study focuses on screening and determining IAA-producing microorganisms from rhizospheric soil samples of crops wheat, maize, and Soyabean. The motive of the research was the application of IAA as a biofertilizer for the above-selected crops. Two bacterial isolates were identified from above rhizospheric soil as IAA producers. Production and Qualitative analysis of IAA was accomplished by use of centrifugation and Salkowaski’s reagent respectively. The effect of IAA as a biofertilizer was further checked by a pot assay to examine the growth of wheat, maize, and Soyabean plants.