International Journal of Biotechnology and Microbiology

International Journal of Biotechnology and Microbiology


International Journal of Biotechnology and Microbiology
International Journal of Biotechnology and Microbiology
2022, Vol. 4, Issue 1
Optimization of bacteriocin production by Lactobacillus fermentum CM 36 and Lactobacillus plantarum CM 114 isolated from camel milk samples

P Upadhyay, Harshada Joshi

Lactic acid bacteria such as lactobacilli are well known to produce a large number of metabolic by-products such as organic acids (like lactic acid, acetic acids etc), H2O2, diacetyl and bacteriocins. Bacteriocins are proteinaceous in nature and play important role in combating pathogenic micro organisms. Present investigation is focused to study the effect of different parameters such as pH, incubation temperature and initial NaCl concentration on bacteriocin production. For this purpose previously isolated and identified camel milk lactobacilli isolates Lactobacillus fermentum CM 36 (LTZ95042) and Lactobacillus plantarum CM 114 (LS992102) were utilized. Production of bacteriocin and effect of parameters such as incubation temperature (30°C, 37°C and 45°C), initial pH (4.0, 7.0 and 9.0) and varying salt (NaCl) concentration (1%, 2% and 3%) on bacteriocin production was determined by using micro dilution method (Daba et al., 1991). Bacteriocin production was measured and represented in the form of arbitrary unit per ml (AU/ml). L. fermentum CM 36 showed maximum bacteriocin titre in MRS broth with incubation temperature 37°C, initial pH 7 without addition of NaCl. On the contrary, L. plantarum CM 114 showed optimum bacteriocin titre in MRS broth at incubation temperature 37°C with initial pH 4 and 7, and 1 and 2% NaCl supplemented in MRS broth. Above mentioned camel milk isolates were proven to have a potential probiotic qualities and study of optimum conditions for enhanced bacteriocin production can play essential role in large scale/ commercial level production.
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How to cite this article:
P Upadhyay, Harshada Joshi. Optimization of bacteriocin production by Lactobacillus fermentum CM 36 and Lactobacillus plantarum CM 114 isolated from camel milk samples. International Journal of Biotechnology and Microbiology, Volume 4, Issue 1, 2022, Pages 11-15
International Journal of Biotechnology and Microbiology