Rakesh Prasad Sah, Rakesh Kumar Mukhia, A D Urhekar
Background: Klebsiella is a gram-negative, encapsulated, facultative anaerobic bacterium which causes hospital and community acquired infections. Klebsiella species has ability to grow in vitro as a biofilm which helps bacteria to survive within host leading to chronic infections as well as development of resistance to antibiotics and disinfectants. Due to development of resistance to antibiotics it is responsible for causing high morbidity & mortality.Objectives: To study biofilm formation in Klebsiella species along with comparison of different phenotypic methods i.e. microscopic determination, Congo Red Agar (CRA) & Tissue culture plate (TCP). Further it was correlated the biofilm formation with antibiotic drug resistance.Material and Methods: A prospective and experimental study was carried out over a period of three years (August 2013 to July 2016). Total 200 isolates of Klebsiella species were tested for the biofilm formation by three different methods i.e. Congo Red Agar, Tissue culture plate and Microscopic determination method. Antibiotic sensitivity was done by Kirby-Bauer Disc diffusion method and the final data was presented using frequency & percentage.Results: Out of 200 isolates of Klebsiella, biofilm formation was detected by Microscopic determination 105 (52.5%), Congo red agar 108 (54%) & Tissue culture plate 119 (59.5%). In the 119 biofilm producing strains detected by TCP method, 94 (79%) strains were MDR (Multi-drug resistant). Highest biofilm producers Klebsiella strains were detected from Accessory devices (83.3%), followed by sputum (76.4%), Endotracheal aspirate (60%), Pus (59.4%), Urine (54.7%), vaginal swabs (50%), Throat swabs (40%), Blood (29.4%) and Stool (25%) by TCP method.Conclusion: TCP is more sensitive & specific method for detection of biofilm and most of the biofilm producing strains were MDR (Multi-drug resistant).