Prevalence of Klebsiella aerogenes and multiantibiotic resistant from two teaching hospitals in Portharcourt
Lemon G Miracle, Lawrence O Amadi, David N Ogbonna
Prevalence and development of multi-antibiotic resistant Klebsiella aerogenes, are part of the major cause of hospital-acquired infections worldwide. This study investigated the multi-antibiotic resistant (MAR) patterns of Klebsiella species, Isolated from FSC from mortuary departments in Rivers state University teaching Hospital and University of Port Harcourt Teaching hospitals for period of 4 months and 7 specimens were collected twice fortnightly. The samples were analyzed by Standard microbiological and Kirby-bauer method, and interpretation of result were according to Clinical Laboratory Standards Institute (CLSI). Reported mean of total coliform counts ranged 5.20 ± 4.89 - 6.62 ± 5.89 x 105, total Klebsiella counts (TSC) 3.4 ± 2.98 - 3.5 ± 3.0x 104cfu/ml from the two mortuaries. The total of 46 isolates consisting of various isolates with relative percentage abundance of Klebsiella sp (82.5%), Citrobacter sp (4.3%), unknown sp (6.5%) & Pseudomonas sp (6.5%).The genus, Klebsiella is the most predominate with relative abundance of: Klebsiella aerogenes (56.5%), Klebsiella oxytoca (21.7%), and Klebsiella pneumoniae (4.3%). The Klebsiella has a higher relative abundance as a single species than Klebsiella pneumoniae & oxytoca isolated from both morgue departments in the two hospitals. The susceptibility pattern of the isolates to antibiotic are Gentamycin (60%)> Ciprofloxacin (25%)> Ofloxacin (10%) and Streptomycin (5%) Respectively. Antibiotic resistant gene evaluation was observed thatKlebsiella aerogenes possess plasmid that carried the following gene: blaCTX-Mtype enzymes of beta-latamase, blaAAC, bla OXA & blaTEM in this study.Klebsiella aerogenes had presented the highest multiantibiotic resistance with a MAR index of more than 1.00. From the above challenge; Approach like educating the public on the danger associating with indiscriminate dispose of corpses and unprotected touching of fresh swabbed corpses and the adverse effect posed to the public.
Lemon G Miracle, Lawrence O Amadi, David N Ogbonna. Prevalence of Klebsiella aerogenes and multiantibiotic resistant from two teaching hospitals in Portharcourt. International Journal of Biotechnology and Microbiology, Volume 3, Issue 2, 2021, Pages 21-28