International Journal of Biotechnology and Microbiology


International Journal of Biotechnology and Microbiology


International Journal of Biotechnology and Microbiology
International Journal of Biotechnology and Microbiology
2021, Vol. 3, Issue 1
Determination of alpha amylase shelf life concentration at increasing temperatures

Emoleila Itoandon, Femi Adams, Ferdinard Okougha, Tijani Azeez, Oluwagbenga Shobowale

An investigation was carried out on the synthesis and characterization of alpha amylase (EC.3.2.1.1). The main aim was achieved using a statistical tool to determine concentration of shelf life at increasing temperatures (oC). The methodology involved a Bacillus subtilis sub sp. construct used for the synthesis of a high efficient alpha amylase (EC.3.2.1.1) and compared to the activities of a wild type Bacillus subtilis. The results showed that the novel Am Bacillus subtilis sub sp. expressed optimal growth density of 0.588mg/ml at the 4th hour as compared to the native strain with 0.464mg/ml at the 6th hour. Assay of sample by the construct recorded 4.2U/ml and 5.2U/ml at pH 5.0 and 60oC respectively as compared to activities of 1.86U/ml and 1.64U/ml recorded at pH 7.0 and 40oC respectively for native Bacillus subtilis. The shelf life concentration of alpha amylase [EC.3.2.1.1] from construct was estimated after 24 hr and 48 hr respectively at different temperatures (oC) using Microsoft Excel 2010 and the results showed that the secretome from novel strain recorded values of 100% at 50oC, 115.9% at 60oC and 88.38% at 70oC after 24hrs and 94.62%, 98.81%, and 88.38% after 48hr respectively. The observations confirmed efficiency of stable alpha amylase [EC.3.2.1.1] by a construct. This further implies that advanced research on alpha amylase (EC.3.2.1.1) is required for high throughput industrial activities and research diversity.
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