Isolation and characterization of biosurfactant producing bacteria from alkaline Lonar Lake
Biosurfactant are the surface active agent produced by the microorganisms and is amphiphilic in nature. They are considered to be a strong alternative to chemical surfactant as low toxicity and biodegradability. Alkaline Lonar Lake is a unique environment and rich in diversity of extremophiles with a source of industrially important microorganisms. In present study 20 sediment samples collected from Lonar lake six different isolates having ability to produce biosurfactant were isolated by enrichment process and continuous sub culturing. Out of them an isolate L2 showed a great potential to produce biosurfactant with respect to reduction in surface tension, oil displacement test, drop collapse test and emulsification index when coconut oil was used as carbon source. The highest reduction in surface tension 20.30 mN/M with 37.58% water oil emulsion formation was recorded when coconut oil was used as carbon source. The result of biochemical characterization and 16S rRNA sequencing showed that the isolate L2 was identified as Bacillus circulans. The isolate showed a characteristic feature for potential to produce biosurfactant and required a further investigation.