Caffeine, a potent phytochemical, obtained from Coffea robusta L is a major component of most famous beverages preferred all over the world. Besides a component of food stuff, it is also a part of many prescription and non-prescription drugs for cold, allergy, pain relievers, Lipolytic, Antimigrane, Antimicrobial agent etc. This study is concerned about extraction, characterization and determination of antimicrobial activity of extracted caffeine. Caffeine extraction was done using ethyl acetate as solvent, giving 0.0457 %. Its conformation was done by Melting Point, λmax and R.F. value determination. The broad spectrum nature of caffeine was confirmed using broth micro dilution method. Since the decades back, potent antimicrobial molecules are always a need of time. Hence using caffeine as a putative molecule can be a way out for the same. Considering other ADR related studies along with cautions about caffeine can point it a presumed biomolecule for various new therapeutic activities.